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Cepas mejoradas para la producción de tracolimus y compuestos análogos mediante el uso de herramientas de ingeniería genética y datos ómicos

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TOES20180406001
Publicado:
18/05/2018
Caducidad:
19/05/2019
Resumen:
Un grupo de investigación de un instituto de biotecnología español ofrece su experiencia y conocimiento en microbiología molecular de la cepa productora de tracolimus (FK506), streptomyces tsukubaensis, para el desarrollo de cepas derivadas mejoradas de alta producción. El instituto busca compañías farmacéuticas para establecer acuerdos de servicio y socios académicos interesados en establecer acuerdos de cooperación en materia de investigación. El grupo de investigación ofrece experiencia y conocimiento en a) técnicas de cultivo microbiano, incluyendo cultivos de fermentación para la producción de metabolitos secundarios y cualificación de productos basada en cromatografía líquida de alta presión (HPLC), b) técnicas de clonación génica e ingeniería genética, c) estudios de regulación transcripcional, d) análisis transcriptómicos, e) proteómica y espectrometría de masas en 2D y f) bioinformática, incluyendo secuenciación del genoma.

Details

Tittle:
Improved strains for production of tacrolimus and analog compounds using genetic engineering tools and omics data
Summary:
A Spanish biotechnological institute offers their experience and knowledge on the molecular microbiology of the producer tacrolimus (FK506) strain, streptomyces tsukubaensis for the development of improved high-producing derivative strains.
Pharma industries are sought for services agreement. Academic partners interested in research cooperation agreement are also sought.
Description:
The organization is a Spanish biotechnological private non-profit research institute, founded in 1993, whose main activities are related to molecular biology and microbial biotechnology. The institution keeps close links with industrial research and innovation. The team covers several techniques and counts with a long experience in scientific research in European funded projects.

Industrial production of tacrolimus and related drugs is hampered by the low production of the wild-type producer strain, Streptomyces tsukubaensis. However, with systems biology and synthetic biology principles it is possible to increase the production levels.

The institute´s research group of molecular microbiology of streptomyces tsukubaensis is seeking pharma companies or academic partners to provide services agreement or to form collaborative research projects in order to achieve research cooperation agreements. For this propose, the group would provide expertise and knowledge in the techniques: a) microbial culture techniques, including fermentation cultures for production of secondary metabolites and high pressure liquid chromatography (HPLC)-based quantification of products; b) gene cloning and genetic engineering techniques, c) transcriptional regulation studies, including electrophoretic-mobility shift assays, footprinting assays, and bioinformatics of binding sites; d) transcriptomic analyses (real time quantitative polymerase chain reaction (RT-qPCR), microarray and ribonucleic acid sequencing (RNA-seq)); e) 2-D proteomics and mass spectrometry, f) bioinformatics, including genome sequencing.

Pharmaceutical companies interested in increasing the production of their own strains, or companies that want to include new production lines, can benefit from this technology. Academic partners interested in collaborating on research projects are also welcome.
Advantages and Innovations:
After the participation in several industrial biotechnology European projects, the institute has accumulated not only knowledge in genetic engineering tools and techniques, but also transcriptomics and proteomics results that would be the base for the production improvement of tacrolimus and other structure-related drugs.

Streptomyces tsukubaensis is one of the species more difficult to be genetically modified. The institute´s molecular microbiology research group counts with proven plasmids and techniques to achieve this porpoise.

The institute research experience and knowledge cover:
- cultivation and handling of this particular strain.
- techniques and tools for genetic engineering and genomic.
- transcriptomics and proteomics data.
Stage of Development:
Concept stage
IPs:
Secret Know-how

Partner sought

Type and Role of Partner Sought:
The biotechnology institute is looking for pharma companies interested in increasing the production of their own strains, or companies starting new production of tacrolimus or related drugs in order to collaborate by a service agreement.

Also academic partners interested in a cooperation research agreement, related with the technique previously described, are welcome.

Client

Type and Size of Client:
R&D Institution
Already Engaged in Trans-National Cooperation:
Si
Languages Spoken:
English
Spanish

Keywords

Technology Keywords:
06003001 Bioinformática
06002006 Biología sintética
06003002 Expresión genética, investigación proteómica
06006011 Fermentación
06002008 Microbiología