Con esta herramienta te facilitamos un acceso a todas las ofertas y demandas de tecnología europeas y a búsquedas de socios para participar en propuestas europeas de I+D publicadas en la red Enterprise Europe Network, pudiendo filtrar los resultados para facilitar las búsquedas más acordes con tus necesidades.

¿Quieres recibir estos listados de oportunidades de colaboración en tu correo de forma periódica y personalizada? Date de alta en nuestro Boletín

Para optimizar los resultados de la búsqueda, se recomienda utilizar términos en inglés.

Ensayo de detección del virus de papiloma humano (VPH) que permite la detección y genotipado simultáneos de 14 cepas diferentes de alto riesto en un tubo cerrado

Resumen

Tipo:
Oferta Tecnológica
Referencia:
13 GB 403U 3SNH
Publicado:
06/01/2015
Caducidad:
17/07/2015
Resumen:
Una pyme británica ha desarrollado un nuevo ensayo que permite la detección y genotipado simultáneos de 14 cepas diferentes de alto riesgo en un tubo cerrado. La tecnología se basa en sondas fluorescentes de doble cadena de diseño especial. Cada sonda, que detecta la presencia de un blanco, tiene una temperatura de fusión distinta, lo que permite distinguirla de otras sondas etiquetadas con el mismo fluoróforo. Este ensayo se emplea para estimación/gestión de riesgos de infecciones persistentes con un genotipo específico, estimación/gestión de riesgos en vacunas y estudios epidemiológicos. Se buscan socios para licenciar y desarrollar conjuntamente la tecnología.

Details

Tittle:
Test for the detection of Human Papillomavirus (HPV) that allows simultaneous detection and genotyping of 14 different high-risk strains in a single-closed tube
Summary:
A UK based SME has developed a novel test that allows the simultaneous detection and genotyping of 14 different high-risk HPV strains in a single-closed tube. It can be used for: risk estimation/management of persistent infections with a specific genotype; risk estimation/management in vaccinations; and epidemiological studies. They are seeking licensing and/or co-development partners.
Description:
A UK based SME has developed a novel test that allows the simultaneous detection and genotyping of 14 different high-risk HPV strains in a single-closed tube. The test was developed as one application of novel technology developed by the company, that is, a novel multiplex detection method, which allows the detection of up to six targets in a single detection channel, using current standard instrumentation. This opens up the prospect of multiplex detection of far more targets in a single closed-tube reaction than the 4-6 currently possible with conventional polymerase chain reaction (PCR) technology.

The technology relies on use of specially designed double stranded fluorescent probes. Each probe, detecting the presence of one target, has distinct melting temperature allowing distinguishing it from other probes labelled with same fluorophore. If a target is present, its corresponding probe is consumed during PCR amplification. Comparing melting profiles of the probes after the reaction reveals which probes have been consumed; this in turn indicates which targets are present in a sample.

The test for the detection of HPV was based on this technology. HPV is a major cause of cervical cancers. This test allows simultaneous detection and genotyping of 14 different high-risk HPV strains in a single-closed tube.
Advantages and Innovations:
Multiplex real-time PCR offers numerous advantages, including time savings, elimination of contamination risk, reduced reagent costs, increased throughput, conservation of precious sample material and reliable results. However, current probe-based methods allow detection of only one target sequence per fluorescence channel. The multiplex fluorescent PCR is therefore limited by the availability of fluorescence dyes and channels; up to five or six targets, depending on particular PCR machines, can be detected simultaneously in the current platforms.

This technology inherits all the benefits of the traditional TaqMan-based real-time PCR at the same time offering several advantages due to its increased multiplexing capabilities:

- increased speed of analysis
- reduced quantity of starting material
- reduced risk of contamination
- reduced overall costs as more targets are analyzed with the same amount of reagents
Stage of Development:
Already on the market
IPs:
Patents granted
CommeR Statunts Regarding IPR Status:
Patent granted in the European Union

Partner sought

Type and Role of Partner Sought:
- Type of partner sought: small- to medium-sized company or research organisation
- Specific area of activity of the partner: Medical diagnostics, genome research, medical testing research, epidemiological research
- Task to be performed by the partner sought: licensing of the existing technology, or adapting/co-developing to new applications
Type of Partnership Considered:
Adaptation to specific needs
Joint further development
License Agreement
Testing of new applications

Client

Type and Size of Client:
Industry SME 11-49
Already Engaged in Trans-National Cooperation:
No

Keywords

Technology Keywords:
06002 Biología / biotecnología
06001003 Citología, cancerología, oncología
06001005 Diagnósticos, diagnosis
06001012 Investigaciones médicas
06001 Medicina, salud humana