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Kits para cultivo celular basados en revestimientos de hidrogel

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TOES20170419001
Publicado:
08/05/2017
Caducidad:
08/05/2018
Resumen:
Centros de investigación españoles han desarrollado nuevos kits para cultivo celular basados en hidrogeles citocompatibles que recubren sustratos rígidos. Estos kits son plataformas mecánicamente robustas y permiten una excelente proliferación celular en la superficie del hidrogel. El despegado de la monocapa celular se realiza sin la necesidad de emplear agentes químicos o biológicos manteniendo la viabilidad del cultivo. Estos kits se han probado con éxito para uso común en biología celular pero se pueden aplicar en la fabricación de constructos biocompatibles más complejos mediante impresión 3D o también como actuadores mecánicos termosensibles. Se buscan empresas del sector de biotecnología interesadas en la licencia de la patente.

Details

Tittle:
Kits for cell culture based on hydrogel coatings
Summary:
Spanish research institutions have developed new kits for cell culture based on cytocompatible hydrogels coated onto stiff substrates. They are mechanically robust platforms. An excellent cellular proliferation on the surface of the hydrogel is achieved. Besides, detachment of a cell monolayer is carried out without the need of chemical or biological agents preserving the viability of cell harvesting. Biotechnology companies are being sought to collaborate through a patent license agreement.
Description:
In cellular biology, traditional methodologies involve the use of aggressive enzymes (most commonly trypsin) or cell scraping. The use of these techniques can damage the harvested cells. The use of thermosensitive systems based on poly-N-isopropylacrylamide (pNIPAm), capable of hosting cells to confluence and subsequent cell sheet detachment, represents a gentler and non-destructive mode of cell harvesting. However, these platforms are difficult to manufacture and economically expensive.
The kits developed by two Spanish research institutions are obtained in a simple process and consist in a thermosensitive hydrogel (based in vinylcaprolactame) or a non-thermosensitive hydrogel (based in vinylpyrrolidone) beared to a rigid polymer substrate. The polymer substrate can be made of hydrophobic materials such as nylon, polystyrene or polycarbonate without the need of surface treatment. These kits have been successfully tested as support for cell culture (muscular, endothelial, skeletal and neural) allowing cell proliferation and/or differentiation. After hosting cells to confluence, a cell monolayer can be detached and transplanted into another surface.
As public research organizations, the applicants of the patent don´t carry out commercial exploitation. The partner sought should be interested in a patent license agreement for industrial escalation and commercial exploitation of this technology.
Advantages and Innovations:
-These kits can be obtained by a simple and industrially scalable method.
-Different types of substrates (e.g. Petri dish or polystyrene multiwell plates) can be used without the need of surface treatments.
-Due to the excellent adhesion of the hydrogel to these substrates is possible to have mechanically robust platforms, easy to handle as required in a cellular biology laboratory.
-Hydrogel may be dried and rehydrated, which is advantageous in terms of storage under clean conditions and delivery.
-Monolayer cell detachment can be carried out by temperature decrease or by simple mechanical agitation.
-These kits have been successfully tested for common use in cellular biology; however they can be used to manufacture more complex biocompatible constructs by 3D printing or also as thermosensitive mechanical actuators.
Stage of Development:
Available for demonstration
IPs:
Patent(s) applied for but not yet granted
CommeR Statunts Regarding IPR Status:
Priority patent application filed suitable for international extension

Partner sought

Type and Role of Partner Sought:
Biotechnology companies specialized in cell culture. In particular, provider of technology products for cellular biology laboratories.

Client

Type and Size of Client:
R&D Institution
Already Engaged in Trans-National Cooperation:
No
Languages Spoken:
English
Spanish

Keywords

Technology Keywords:
06002002 Biología celular y molecular
02007014 Plastics, Polymers
05001004 Química orgánica