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Marcadores de metilación para identificación de fluidos corporales y tejidos en evidencia traza forense

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TODE20150312001
Publicado:
24/03/2015
Caducidad:
23/03/2016
Resumen:
Un laboratorio y una pyme alemanes han desarrollado conjuntamente un nuevo procedimiento para identificar fluidos corporales mediante metilación diferencial del ADN. El proceso puede esclarecer el curso de los acontecimientos en el lugar donde se produce un delito, especialmente en el caso de una agresión sexual. Puesto que la metilación es específica del tejido, es posible caracterizar de forma precisa una línea celular cuantificando el grado de metilación en estas posiciones. Las ventajas incluyen fiabilidad, claridad y estabilidad. Se buscan autoridades contra la delincuencia, laboratorios forenses y centros de investigación con el fin de establecer acuerdos comerciales con asistencia técnica.

Details

Tittle:
Methylation markers for the identification of body fluids and tissues from forensic trace evidence
Summary:
A German laboratory and a German SME have co-developed a novel procedure to identify body fluids through differential DNA-methylation. The procedure can clarify the course of events at a criminal site, especially with regard to sexual assaults. Advantages include its reliability, clarity and stability. Crime-fighting authorities, forensic laboratories and research institutions are sought for commercial agreements with technical assistance.
Description:
The analysis of a biological trace found at the crime site ought to help to identify the perpetrator and to clarify the course of events. The most efficient tool for getting information about the identity of the involved persons is the genetic fingerprint, whereas the biological nature and composition of the material very often elucidates the course of events. In contrast to the very clear and worldwide standardized situation for the genetic fingerprint, the identification of body fluids from forensic traces is still a severe problem Typically, a biological trace at the crime site is exposed to various physical impacts such as water, air and sunlight or biochemical and microbiological impacts. Most of them eventually destroy the biological components that are being used to identify a body fluid. A widely used procedure is the stepwise analysis of the material. Unfortunately, the unspecific tests applied as a first step require much of the sample material or even destroy the DNA which is necessary for the genetic fingerprint. Almost all subsequently used specific tests turned out to be insufficient.

A German laboratory and a German SME have developed a procedure which uses the phenomenon of differential DNA-methylation which is a natural tool of gene regulation and cell specific gene expression. Since methylation is tissue specific, a cell line can be precisely characterized by quantifying the degree of methylation at such positions.
Epigenetic markers have been identified with regard to the body fluids venous blood, menstrual blood, vaginal fluid, seminal fluid, saliva, skin and sex determination.
The marker systems have one marker being methylated in the target fluid and unmethylated in the remaining fluids and a second one that is unmethylated in the target fluid but methylated in the others. This principle allows an even better analysis of complex mixtures, because admixtures of any other fluid produce a methylation signal.
The method allows the identification of the target fluid in mixtures of other body fluids.
For the final determination of the methylation degree the SNuPE-method has been used. Markers have been analyzed in samples of menstrual blood (n=96), venous blood (n=100), vaginal fluid (n=55), saliva (n=100) and semen fluid (n=91).

The newly developed test procedure has been statistically validated. The stability of the detection procedure is proved in various simulated forensic surroundings according to standardized conditions. The developed markers have been applied successfully in criminal proceedings, the new procedure turned out to be crucial for the outcomes.

Partners from crime-fighting authorities (especially sexual assaults), forensic laboratories and research institutions are sought for commercial agreements with technical assistance.
Advantages and Innovations:
- New method allows for a clear and precise determination and identification of body fluids which is not possible with traditional DNA fingerprints
- High reliability and stability of detection
- Allows for easier identification of target fluid in mixtures of other body fluids
Stage of Development:
Already on the market
IPs:
Patents granted
CommeR Statunts Regarding IPR Status:
German and European Patent

Partner sought

Type and Role of Partner Sought:
Crime-fighting authorities (especially sexual assaults), forensic laboratories and research institutions are sought to apply the developed technology.
Necessary knowhow for the application and technology transfer will be supplied by the German partners.

Client

Type and Size of Client:
R&D Institution
Already Engaged in Trans-National Cooperation:
Si
Languages Spoken:
English
German
Russian

Keywords

Technology Keywords:
06002002 Biología celular y molecular
06002001 Bioquímica / biofísica
06002007 Ensayos in vitro, experimentos
06002008 Microbiología
06001009 Terapia genética - ADN