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Método analítico de detección ultrarrápida de proteínas de leche de vaca en productos de soja mediante cromatografía líquida de alta eficacia

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TOES20150928005
Publicado:
30/11/2015
Caducidad:
30/11/2016
Resumen:
Un grupo de investigación español especializado en análisis ha desarrollado un procedimiento ultrarrápido de detección de proteínas de leche de vaca en productos lácteos de soja. Este método, basado en cromatografía líquida de alta eficacia, representa un avance en el control de calidad rutinario de estos productos. El método de detección simultánea de proteínas de soja y proteínas de suero de leche de vaca mediante cromatografía líquida de alta eficacia de perfusión en fase inversa se realiza con un cromatógrafo líquido de alta presión. Se buscan socios con el fin de establecer acuerdos de licencia o comercialización con asistencia técnica.

Details

Tittle:
Analitical method for ultrafast detection of protein of cow´s milk in soybean products by high-performance liquid chromatography.
Summary:
A spanish research group working on analytics has developed an ultrafast procedure for detection of whey protein of cow´s milk in dairy-type products of soybean. This method, done by high-performance liquid chromatography, represents a breakthrough for routine quality control analysis of these products. The group is seeking for commercial agreements with technical assistance or license agreements.
Description:
Food related allergies have increased in recent years. One of this allergies is to whey protein of cow´s milk. This have led to a new line of product dairy-type products of soybean. But, since many producers manufacture both products, cross-contamination can occur. Until recently, there has not been a method to characterized and quantitatively determined both types of proteins, nor being able to do it in a few minutes.

A Spanish research group working on analytics has addressed this problem and developed an ultrafast procedure for detection of whey protein of cow´s milk in dairy-type products of soybean. The analytical method which allows the simultaneous determination of soybean proteins and whey protein of cow´s milk by perfusion reversed-phase high-performance liquid chromatography (HPLC) is carried out in a high pressure liquid chromatograph allowing to perform gradients of mobile phase composition and it has coupled a detector for ultraviolet-visible absorption (UV-Vis) of variable wavelength.
The chromatographic column used is a column with a filling of perfusion in reversed phase mode. The separation is carried out at a temperature of the column constant and equal to 60ºC and the detection is performed at a wavelength of 254 nm.
The group is seeking for commercial agreements with technical assistance or license agreements. They have interest both in manufacturers of dairy-type products of soybean and administration.


Advantages and Innovations:
Innovations:

Although the whey protein of cow´s milk and soybean proteins have been characterized and quantitatively determined separately using different techniques, until recently there has not been separated simultaneously both types of proteins, nor in as little time (a few minutes).


Advantages:

- This method can be performed with basic instrumentation and therefore, it is accessible to the majority of the analytical laboratories.
-The gradient used allows the simultaneous separation of the soybean protein and the whey protein of cow´s milk.
- It is a simple method because it does not require a complex previous treatment of the sample or patterns.
- It allows the ultrafast separation and determination of these two types of proteins, making it ideal for industrial application.
- It allows the detection of adulteration due to the addition of whey proteins of cow´s milk to dairy-type commercial products made from soy protein isolate.
- It allows simultaneously and separately to quantify soybean proteins and whey proteins of cow´s milk.
- It allows to determine the content of soybean protein and whey proteins from cow´s milk in samples with other additives containing nitrogen and for which existing methods for the determination of total nitrogen, do not allow to specify the protein content of the sample.
Stage of Development:
Already on the market
IPs:
Patents granted
CommeR Statunts Regarding IPR Status:
National patent. Spanish

Partner sought

Type and Role of Partner Sought:
The group is seeking for commercial agreements with technical assistance or license agreements patent.
Implementation of the developed technology from the technical knowledge transfer. Technical preparation, development or adaptation of the technology to specific needs of the business.
Technical cooperation mainly in areas of quality control and food safety.
It could be interesting for both, the manufacturer and the administration, to certify that regulators endorse this content.

Client

Type and Size of Client:
University
Already Engaged in Trans-National Cooperation:
Si
Languages Spoken:
English
Spanish

Keywords

Technology Keywords:
08002002 Microbiología / toxicología / control de calidad de alimentos
08002001 Métodos de análisis y detección
10001006 Protección contra intoxicaciones