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Microscopio holográfico de fluorescencia para observar funciones y estructuras de microobjetos translúcidos


Oferta Tecnológica
Una universidad checa ha lanzado un microscopio holográfico de fluorescencia para formación de imágenes de objetos translúcidos capaces de emitir radiación luminiscente. El microscopio permite estudiar simultáneamente la función y estructura de microobjetos vivos, como células y microorganismos, así como su reacción a los cambios del entorno. También se puede utilizar para formar imágenes de microobjetos técnicos. La universidad busca un fabricante de microscopios con el fin de establecer acuerdos de licencia.


Fluorescence holographic microscope for observing functions and structures of translucent (living) micro-objects. Looking for licensee.
Czech well-known university introduces fluorescence holographic microscope designed for imaging of translucent objects capable of emitting luminescence radiation. The microscope allows to study simultaneously the function and structure of living micro-objects such as cells and microorganisms as well as their reaction to environmental changes. It can also be used for imaging of technical micro-objects. The university is looking for a microscope manufacturer interested in licensing agreement.
The fluorescence holographic microscope was designed and constructed by skilled and experienced team of scientists focused on research, development, and applications of innovative microscopy technologies. This team has developed several other holographic microscopes. One of their previously developed holographic microscopes is currently being put onto the market in collaboration with a global manufacturer of scientific instruments.
Fluorescence is a widely used technique of imaging in biomedical microscopy, because it enables high contrast, colour coded observing/imaging of transparent objects like cells or tissues. An observed sample needs to contain particles (fluorochromes) able to emit specific light (fluorescence). It is not always necessary to dye the samples which could negatively influence the sample itself (e.g. cell vitality). Fluorescence light emitted by particles present naturally in the sample, so called autofluorescence, can be used instead.

Compared to the existing fluorescence microscopes, this microscope uses two opposing objective lenses which both are focused to the same point and it allows to study the function and structure of living micro-objects simultaneously. Light emitted by the object is collected simultaneously by both lenses and it is detected by a single detector. The detector captures a pattern called hologram. Hologram is processed numerically and two image modes are obtained: amplitude and phase images.

"Amplitude image" allows to reconstruct the spatial distribution of fluorescent-labelled structures (e.g. nuclei of cells, cytoskeleton) or substances (e.g. proteins or other molecules of interest) within the observed object, similarly to the existing fluorescence microscopes. On the other hand, "phase image" provides quantitative information about the spatial distribution of mass density within the observed object.

Suitable objects can be both plant and animal cells, clusters of cells, microorganisms, but also technical micro-objects.

The microscope can be used for example for observation of live cell cultures (microorganisms) and their responses to various external stimuli (pressure, temperature, toxic agents, drugs ...).

This device can therefore be used for quantitative, time-course monitoring of changes in spatial distribution of cellular mass in relation to the external conditions. This information is a bonus to the commonly available intensity of fluorescence radiation that reveals the cellular structure.

The Czech university is looking for a microscope manufacturer (manufacturer of scientific instruments) interested in licensing agreement. This agreement will include a research cooperation, if necessary, to support the manufacturer in market introduction of the microscope.
Advantages and Innovations:
- The first (amplitude) image visualizes optical section of the intensity of fluorescence radiation. The full-field optical section is obtained from a single hologram captured in one-shot, no lateral scanning across the field of view (known from a confocal microscopy) is needed and a high-speed imaging is possible. This high-speed imaging leads to reduction of image noise (caused by moving of the objects and surrounding environment).

- The second (phase) image reveals the 3D spatial distribution of mass density within the sample (live cell). This is not possible to achieve with classical holographic microscopy, where only the integral value of mass density along the optical axis is obtained for each pixel.

- Optical sectioning effect (axial resolution) may be enhanced compared to a standard confocal microscopy and the thickness of the optical section is constant across the field of view (unlike light-sheet microscopy).
Stage of Development:
Available for demonstration
Patent(s) applied for but not yet granted
CommeR Statunts Regarding IPR Status:
CZ utility model granted
CZ patent applied for
PCT application applied for

Partner sought

Type and Role of Partner Sought:
- Type of partner sought: SME, industry, MNE

- Specific area of activity of the partner: Manufacturer of microscopes

- Task to be performed by Parner sought: Market implementation of the microscope based on license agreement


Type and Size of Client:
Already Engaged in Trans-National Cooperation:
Languages Spoken:


Technology Keywords:
09003 Sistemas electrónicos de medida
09001007 Tecnología óptica relacionada con la realización de medidas