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Novel luminescent probes of lanthanide complexes for sensing of biomolecules

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TOES20200512001
Publicado:
03/07/2020
Caducidad:
04/07/2021
Resumen:
A research institution and a university from Spain have developed a new family of antenna chromophores able to sensitize effectively luminescence of lanthanides to be applied as labelling probes for biomolecules. Industrial partners specialized in probes for biological research are being sought for patent licensing and/or research collaboration.

Details

Tittle:
Novel luminescent probes of lanthanide complexes for sensing of biomolecules
Summary:
A research institution and a university from Spain have developed a new family of antenna chromophores able to sensitize effectively luminescence of lanthanides to be applied as labelling probes for biomolecules.

Industrial partners specialized in probes for biological research are being sought for patent licensing and/or research collaboration.
Description:
Luminescent lanthanide probes are emerging as powerful tools in in vitro labelling of biomolecules and nanoparticles for monitoring of bioassays in diagnostics or drug discovery.
Use of sensitizing chromophores or antennas as ligands in a lanthanide complex allows increasing luminescence intensity of the lanthanide ion. However, its current application is limited due to the difficulty to label the biomolecule in an easy way.
The authors have developed two families of antenna ligands able to bind efficiently to biomolecules and sensitize luminescence emission of lanthanide cations, especially Europium III cations (Eu3+) and Terbium III (Tb3+).
Assays performed with different peptides and proteins labelled with lanthanide complexes using the developed antennas showed between 2,000 and 12,000 fold luminescence increase with Tb3+ complexes and between 8,000 and 68,000 fold with Eu3+ complexes.
Similarly, nanoparticles labelled with these lanthanide complexes have been used efficiently for tumour cells imaging.

The Spanish research organizations have broad experience in the development of fluorescent biosensors with proteomic applications. They are searching for an industrial partner interested in the development and commercialization of specific applications using these probes, under a patent license agreement. The research institutions could collaborate with the company through research collaboration to develop such applications.
Advantages and Innovations:
· Easy and efficient generation of luminescent lanthanide sensors using novel antennas able to increase the intensity of the lanthanide´s fluorescence emission once bonded to the target.
· Effective implementation in the luminescent labelling of biomolecules and nanoparticles in vitro.
· Easy target labelling both in solution and in solid phase.
· Advantages over organic fluorophores and fluorescent protein are:
- Long luminescence lifetime (µs-ms) erasing interferences by autofluorescence of the biological systems and increasing signal-to-noise ratio, hence sensitivity.
- Sharp emission peaks with narrow bandwidth that do not overlap.
- Large Stokes´ shift avoiding self-absorption.
· They allow simultaneous use of different lanthanide probes to detect several targets (multichannel detection).


Stage of Development:
Prototype available for demonstration
IPs:
Trade Marks
CommeR Statunts Regarding IPR Status:
Two Spanish priority patent applications filed suitable for international extension

Partner sought

Type and Role of Partner Sought:
Suppliers of research tools for biochemical and biological laboratories, interested in the development and commercialization of the reported probes under a license agreement, are sought.

Research cooperation agreement: The partner and the institutions could undertake a joint effort to further develop this
technology, obtaining specific applications addressed to the market needs.

Client

Type and Size of Client:
R&D Institution
Already Engaged in Trans-National Cooperation:
No
Languages Spoken:
English
Spanish

Keywords

Technology Keywords:
06002002 Biología celular y molecular
06002001 Bioquímica / biofísica
06002007 Ensayos in vitro, experimentos