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Nueva técnica de reacción en cadena de la polimerasa cuantitativa (qPCR) para diagnóstico que aumenta la sensibilidad de ensayos normales mediante el uso de iniciadores anidados múltiples

Resumen

Tipo:
Oferta Tecnológica
Referencia:
13 GB 403U 3SNK
Publicado:
06/01/2015
Caducidad:
17/07/2015
Resumen:
Una pyme británica ha desarrollado una nueva técnica de reacción en cadena de la polimerasa (PCR) que utiliza múltiples iniciadores anidados en una reacción rápida de un tubo que aumenta la sensibilidad de los ensayos normales de reacción en cadena de la polimerasa (qPCR) cuantitativa. Mientras que la técnica PCR convencional utiliza un par de iniciadores, esta técnica se basa en múltiples iniciadores. Los iniciadores anidados flanquean la región de interés. Se buscan empresas o centros de investigación interesados en licenciar la tecnología o adaptarla a nuevas aplicaciones.

Details

Tittle:
Novel quantitative polymerase chain reaction (qPCR) diagnostic technique that increases the sensitivity of normal assays by using multiple nested primers
Summary:
A UK SME has developed a new testing polymerase chain reaction (PCR) technique which uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative polymerase chain reaction (qPCR) assays. They have used the technology to develop two new PCR enhancement technologies and are seeking companies or research institutes interested in licensing the technology or adapting it to new uses.
Description:
Quantitative polymerase chain reaction (qPCR) assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.

The UK company has developed a new technique, which uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays.

Conventional PCR uses a pair of primers and therefore produces a maximum of a 2-fold increase in amplicon per amplification cycle. The company has developed a modification to PCR that uses more than one pair of primers. Nested primers are designed that flank the region of interest; when the outer and inner primers are extended, the extended strand of the outer primer causes displacement of the extended strand produced from the inner primer.

The company has developed two new products utilizing this PCR enhancement technology:
- Generic mastermix - containing hot-start DNA polymerase with strand displacement activity, Deoxynucleotide Triphosphates (dNTPs) and optimized buffer.
- Denguetest - real-time assay for detection of all 4 serotypes of dengue virus, the causative agent of dengue fever.
Advantages and Innovations:
This technology allows more than two amplicons to be produced after each amplification cycle and therefore greater sensitivity and increased speed of assay. Sensitivity was increased by approximately 10-fold in a proof-of-principle test on dengue virus sequence.

An increase in the sensitivity of amplification assays would be beneficial in diagnostic uses for virus detection where there is a low starting copy number or where sensitivity is lost by PCR inhibitors present in crude extracts.

Another advantage of using multiple primers for viral detection lies in decreased sensitivity of such set-up to random mutations frequently occurring in viral genomes. It is much less likely that in a particular subtype of virus mutations would occur in both outer and inner primer binding sites and therefore amplification of the target would still occur.
Stage of Development:
Already on the market
IPs:
Patents granted
CommeR Statunts Regarding IPR Status:
Patent granted in the USA.

Partner sought

Type and Role of Partner Sought:
- Type of partner sought: company or research institute

- Specific area of activity of the partner: medical diagnostics, medical research

- Task to be performed by the partner sought: licensing of existing technology or adaptation or co-developed towards new applications

Client

Type and Size of Client:
Industry SME 11-49
Already Engaged in Trans-National Cooperation:
No

Keywords

Technology Keywords:
06002 Biología / biotecnología
06001003 Citología, cancerología, oncología
06001005 Diagnósticos, diagnosis
06001012 Investigaciones médicas
06001 Medicina, salud humana