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Nuevo método de reacción en cadena de la polimerasa multiplex (PCR) para detección de hasta cuatro blancos en un canal de detección único mediante el uso de instrumentos estándar

Resumen

Tipo:
Oferta Tecnológica
Referencia:
13 GB 403U 3SNI
Publicado:
11/02/2015
Caducidad:
30/08/2015
Resumen:
Una pyme británica ha desarrollado un nuevo método de detección que ofrece numerosas posibilidades de aplicación en diagnósticos médicos. La tecnología se basa en sondas fluorescentes de doble cadena de diseño especial. Cada sonda, que detecta la presencia de un blanco, tiene una temperatura de fusión distinta, lo que permite distinguirla de otras sondas etiquetadas con el mismo fluoróforo. El método PCR (reacción en cadena de la polimerasa) permite detectar hasta cuatro blancos en un canal de detección único mediante el uso de instrumentos estándar. Se buscan empresas y centros de investigación interesados en desarrollar conjuntamente la tecnología y buscar nuevas aplicaciones.

Details

Tittle:
Novel multiplex polymerase chain reaction (PCR) detection method which allows the detection of up to four targets in a single detection channel using current standard instrumentation
Summary:
A UK SME has developed a novel detection method which has multiple possible applications in medical diagnostics. The PCR method allows the detection of up to four targets in a single channel, using current standard instrumentation. This opens up the prospect of multiplex detection of far more targets in a single closed-tube reaction than the 4-6 currently possible. They are seeking companies or research organisations to co-develop this technology to novel applications, or for licensing.
Description:
UK SME has developed a novel detection method which has multiple possible applications in medical diagnostics. The technology relies on use of specially designed double stranded fluorescent probes. Each probe, detecting the presence of one target, has distinct melting temperature allowing distinguishing it from other probes labelled with same fluorophore. If a target is present, its corresponding probe is consumed during PCR amplification. Comparing melting profiles of the probes after the reaction reveals which probes have been consumed; this in turn indicates which targets are present in a sample.

The company has already developed a test for the detection of Human Papillomavirus (HPV) based on this technology (which is itself a separate technology profile - 13 GB 403U 3SNH). HPV is a major cause of cervical cancers. This test allows simultaneous detection and genotyping of 14 different high-risk HPV strains in a single-closed tube. It can be used for: risk estimation/management of the persistent infection with a specific HPV genotype; risk estimation/management in HPV vaccinations; and epidemiological studies.
Advantages and Innovations:
Multiplex real-time PCR offers numerous advantages, including time savings, elimination of contamination risk, reduced reagent costs, increased throughput, conservation of precious sample material and reliable results. However, current probe-based methods allow detection of only one target sequence per fluorescence channel. The multiplex fluorescent PCR is therefore limited by the availability of fluorescence dyes and channels; up to five or six targets, depending on particular PCR machines, can be detected simultaneously in the current platforms.

This technology inherits all the benefits of the traditional TaqMan-based real-time PCR at the same time offering several advantages due to its increased multiplexing capabilities:

- increased speed of analysis
- reduced quantity of staring material
- reduced risk of contamination
- reduced overall costs as more targets are analyzed with the same amount of reagents
Stage of Development:
Already on the market
IPs:
Patents granted
CommeR Statunts Regarding IPR Status:
Patent granted in the European Union

Partner sought

Type and Role of Partner Sought:
- Type of partner sought: companies or research institutions
- Specific area of activity of the partner: development of novel diagnostic applications
- Task to be performed by the partner sought: co-development of technology to new applications or licensing of existing technology
Type of Partnership Considered:
Adaptation to specific needs
Joint further development
License Agreement
Testing of new applications

Client

Type and Size of Client:
Industry SME 11-49
Already Engaged in Trans-National Cooperation:
No

Keywords

Technology Keywords:
06002 Biología / biotecnología
06001003 Citología, cancerología, oncología
06001005 Diagnósticos, diagnosis
06001012 Investigaciones médicas
06001 Medicina, salud humana