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Nuevo sensor molecular

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TODE20180418001
Publicado:
02/05/2018
Caducidad:
02/05/2019
Resumen:
Una universidad alemana ha desarrollado un sensor molecular que detecta moléculas de adenosín monofosfato cíclico (cAMP) con mayor sensibilidad y una respuesta más rápida que los sensores disponibles en la actualidad. El adenosín monofosfato cíclico es un segundo mensajero importante en numerosos procesos biológicos. Este sensor es una herramienta de investigación para empresas de farmacología. Otras ventajas incluyen la afinidad nanomolar, cinética de unión/desunión rápida, expresión y purificación en altas cantidades de células procariotas, buena expresión en células eucariotas, medición de los niveles de cAMP en solución in vitro, in cellulo e in vivo o ausencia de interacción con vías moleculares de células eucariotas. La universidad busca socios con el fin de establecer acuerdos de licencia.

Details

Tittle:
Novel molecular sensor.
Summary:
A German university has developed a molecular sensor that detects cyclic adenosine monophosphate (cAMP) molecules with a higher sensitivity and a faster response than currently available sensors. The sensor is a research tool for companies active in pharmacology. The university offers a license agreement.
Description:
Cyclic adenosine monophosphate (cAMP, cyclic AMP) is a second messenger important in many biological processes. Cyclic AMP is a key molecule in 2nd messenger cell signaling. Great effort has been taken to develop sensors that allow for noninvasive assessment of cAMP concentrations in living cells. Genetically encoded biosensors hold great promise to allow spatial and temporal measurements of cAMP concentrations in living cells. Yet, the sensors available up to now are hampered by the low affinity of the cAMP binding moieties employed.

A German university has now found a new biosensor that shows a higher sensitivity and a faster response in order to detect cAMP molecules. The inventors of the new sensor identified the cAMP nucleotide-binding domain of the bacterial MlotiK1 channel as superior cAMP sensing moiety for a FRET-based cAMP sensor. Sandwiched between a blue and a yellow fluorescent protein, this motif exhibits a nanomolar affinity and allows for real-time measurement of intracellular cAMP concentration. The inventors were able to monitor the cAMP dynamics in different cell types, e.g. HEK293 cells, but also in flagella of sperm, thereby revealing the spatio-temporal distribution of cAMP controlling flagellar movement. This work (please see picture below) proves the applicability of the presented cAMP sensor for the investigation of cAMP-related biological questions.

The university offers a license agreement to companies active in pharmacology.
Advantages and Innovations:
The overall advantage and innovation of this biosensor that it is characterised by higher sensitivity and faster response than sensors presently available. Other advantages on a more specific level are:
· Nanomolar affinity
· Fast binding/unbinding kinetics
· Expressed and purified in high amounts from prokaryotic cells
· Well expressed in eukaryotic cells.
· Measurement of cAMP levels in solution in vitro, in cellulo, and in vivo
· No interaction with molecular pathways of eukaryotic cells
· Amenable to subcellular targeting
Stage of Development:
Already on the market
IPs:
Patent(s) applied for but not yet granted,Patents granted
CommeR Statunts Regarding IPR Status:
A WO (World International Property Organisation) patent has been filed and German patent has been granted.

Partner sought

Type and Role of Partner Sought:
The university offers a license agreement to companies active in pharmacology.
A licensee has the option to sell the invention directly to his clients. Another partner could be one that designs and sells molecular screening systems and could use this invention to extend their portfolio.

Client

Type and Size of Client:
University
Already Engaged in Trans-National Cooperation:
Si
Languages Spoken:
English
German

Keywords

Technology Keywords:
06002002 Biología celular y molecular
05002001 Biosensores
06002009 Diseño molecular
06002005 Ingeniería genética