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Proceso de inserción de genes en bacterias

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TOES20150715002
Publicado:
15/09/2015
Caducidad:
27/08/2016
Resumen:
Investigadores del área de microbiología de una universidad española han desarrollado y caracterizado un sistema de transferencia de genes mediante minitransposición que se utiliza para la inserción estable de genes de ADN ajeno en el genoma de bacterias. Se busca una compañía de biotecnología que ofrezca servicios de ingeniería genética a medida con el fin de licenciar la patente o establecer acuerdos de cooperación técnica o investigación.

Details

Tittle:
Procedure for gene insertion into bacteria
Summary:
Researchers in the area of microbiology at a Spanish university have developed and characterized a system for gene transfer by mini transposition useful for stable gene insertion of foreign DNA into the genome of bacteria. A biotechnological company offering customized genetic engineering services interested in a patent license or in a research or technical cooperation agreement are being sought.
Description:
The minitransposon-based insertion systems have proved to be the simplest and fastest mechanisms for inserting DNA fragments into a host chromosome.

In comparison with other methods, the novelty of this new procedure resides in the mechanism for integration of the genetic material in the host, avoiding unstable plasmids or low-frequency recombination processes. As DNA integration usually proceed in monocopy, plausible toxicity effects produced by foreign DNA or multicopy DNA integration are avoided.

Integration frequencies obtained with the method are 3-fold higher than the ones obtained for similar trans-acting transposase procedures.

This process uses the in trans action of the insertion sequence (IS) ISPpu12, a strongly regulated IS after conjugative interaction stimulus, to insert genetic material of interest in the host bacteria.
Taking advantage of such activation only during conjugation, inverse repeats (IR) sequence, IR-DNA fragment-IR structures of up to 10-kb can be easily inserted in a stable way in the chromosome of the bacteria permitting two or three consecutive processes.

The process has already been well characterized and the tools necessary for applying it have been synthesized and tested. The new process might be of interest to companies offering a customized genetic engineering service to their clients.
Advantages and Innovations:
The process permits host bacteria achieve almost any metabolic process or phenotype which is codified in a known DNA fragment. It is a very fast, easy and reliable way for inserting large genetic fragments of interest (up to 10 kb) in a host bacterium.

The system can work without antibiotic reporter genes to screen for transference of genetic material which allows a considerable reduction in costs.

Frequencies of genetic acquisition are high (between 10-4-10-6, depending on the size of genetic insert), avoiding alternative and non-desirable processes, and saving screening time.

The genetic material acquired is integrated in the chromosome of the bacteria which means a stable insertion.

Insertion is produced mainly in one copy avoiding toxicity and the decrease of hosts´ fitness and growth rates.
Stage of Development:
Field tested/evaluated
IPs:
Patents granted

Partner sought

Type and Role of Partner Sought:
A biotechnological company offering customized genetic engineering services interested in a patent license or in a research or technical cooperation agreement.

Client

Type and Size of Client:
University
Already Engaged in Trans-National Cooperation:
No
Languages Spoken:
English
Spanish

Keywords

Technology Keywords:
06002005 Ingeniería genética