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Producción de proteínas recombinantes en la superficie celular de bacterias
Resumen
Tipo:
Oferta Tecnológica
Referencia:
TODE20160617001
Publicado:
23/06/2016
Caducidad:
23/06/2017
Resumen:
Un hospital universitario alemán ha desarrollado un nuevo enfoque para la producción de proteínas recombinantes que se emplea en la producción de reactivos para diagnósticos y tratamientos. Estas proteínas poseen una estructura plegable natural y la misma actividad biológica que las proteínas naturales. La tecnología permite la expresión de proteínas de interés en la superficie celular de bacterias, evitando la acumulación citoplasmática y periplasmática. El hospital busca licenciatarios del sector de biotecnología.
Details
Tittle:
Production of recombinant protein on the cell surface of bacteria
Summary:
A German university hospital developed a novel approach for the production of recombinant proteins, that can be used for the production of reagents for diagnostics and therapy. These proteins have a natural folding structure and the same biological activity as a natural protein. Licensees from the biotech sector are sought.
Description:
The expression and production of recombinant proteins in bacteria, such as E. coli is a long standing and well known procedure. However, expression in bacterial systems is limited due to cytoplasmic accumulation of the protein of interest (POI). Even though, various attempts have been made to prevent the accumulation of the desired protein in inclusion bodies, there is to date no satisfactory solution available to overcome this problem.
The invention by a German university hospital enables the expression of POI on the cell surface of bacteria, thereby preventing the cytoplasmic and periplasmic accumulation. This is achieved by using an expression vector comprising the bacterial cell-surface protein FimH and the POI. Bacterial expression of this fusion protein leads to the localization of the protein on the cell surface. Thus, the fusion protein may be isolated from the surface of the bacterial cells or, alternatively, be isolated from the bacterial cell medium subsequent to secretion.
Thus, this novel strategy allows the production of recombinant proteins with a natural folding structure and the same biological activity as a natural protein. They can be used for the production of reagents that are suitable for diagnostics and therapy.
The The university offers licenses to industrial partners from the field of biotechnology, interested in implementing the new method.
The invention by a German university hospital enables the expression of POI on the cell surface of bacteria, thereby preventing the cytoplasmic and periplasmic accumulation. This is achieved by using an expression vector comprising the bacterial cell-surface protein FimH and the POI. Bacterial expression of this fusion protein leads to the localization of the protein on the cell surface. Thus, the fusion protein may be isolated from the surface of the bacterial cells or, alternatively, be isolated from the bacterial cell medium subsequent to secretion.
Thus, this novel strategy allows the production of recombinant proteins with a natural folding structure and the same biological activity as a natural protein. They can be used for the production of reagents that are suitable for diagnostics and therapy.
The The university offers licenses to industrial partners from the field of biotechnology, interested in implementing the new method.
Advantages and Innovations:
Completely new strategy
Recombinant protein with
- Natural folding structure
- Natural biological activity
Expression on the bacterial cell surface
No cytoplasmic or periplasmic protein accumulation
Recombinant protein with
- Natural folding structure
- Natural biological activity
Expression on the bacterial cell surface
No cytoplasmic or periplasmic protein accumulation
Stage of Development:
Under development/lab tested
IPs:
Patent(s) applied for but not yet granted
Partner sought
Type and Role of Partner Sought:
Innovative partners from biotechnology industry involved in diagnostic and therapeutic research are sought as licensees.
Client
Type and Size of Client:
University
Already Engaged in Trans-National Cooperation:
Si
Languages Spoken:
English
German
German
Keywords
Technology Keywords:
06002002 Biología celular y molecular
06002004 Ingeniería de proteínas
06001015 Productos farmacéuticos / medicamentos
06001018 Virus, virología / antibióticos / bacteriología
06002004 Ingeniería de proteínas
06001015 Productos farmacéuticos / medicamentos
06001018 Virus, virología / antibióticos / bacteriología
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