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Universidad húngara ofrece un nuevo receptor fotoactivo y fluorescente

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TOHU20160914002
Publicado:
19/10/2016
Caducidad:
19/10/2017
Resumen:
Un equipo de investigación de una universidad húngara ha desarrollado un receptor antagonista AMPA fotoactivo que se enlaza covalentemente al objetivo y se vuelve fluorescente después del enlace. La señal fluorescente permite controlar la eficiencia de inhibición, cuantificación, localización y estabilidad. Los receptores se utilizan para llevar a cabo experimentos de aprendizaje e investigación de memoria. El potencial de mercado se encuentra en el campo de biología molecular. El equipo de investigación busca socios con el fin de establecer acuerdos de joint venture, licencia, investigación y cooperación técnica.

Details

Tittle:
Hungarian university offers a novel photoactive and fluorescent receptor
Summary:
A Hungarian university team invented ABQX which is a photoactive AMPA receptor antagonist that can be covalently crosslinked to the target and it becomes fluorescent upon crosslinking. The fluorescent sign allows controlling inhibition efficiency, quantification, localization and stability. Experiments can be performed with the receptors in learning and memory research. The potential market is cellular and molecular biology. They look for joint venture, licence, research and technical agreement.
Description:
The research team works in the framework of one of the largest Hungarian university. They deal with cellular and molecular biology issues.

More than 90% of the synapses in the brain use glutamate as neurotransmitter, and contain mostly AMPA (alfa-amino-3-hidroxi-5-metil-4-izoxazolpropion-acid) type glutamate receptors. These receptors provide fast stimulating neurotransmission in the brain in an activity-dependent manner, providing neuronal plasticity that is a key factor in learning and memory. Change in synaptic strength is mostly due to the traffic of postsynaptic AMPA receptors that are regulated by synaptic activity. This constant postsynaptic AMPA receptor traffic allows the regulated change of AMPA receptor quantity in the synapse, leading to synaptic plasticity.

ANQX (non-fluorescent 6-azido-7-nitro-1,4-dihydroquinoxaline-2,3-dione) is a non cell permeable AMPA receptor antagonist, which was developed previously for the direct and quantitative study of synaptic plasticity in living neurons.
ANQX allows the photoinactivation of cell surface AMPA receptors by forming covalent complexes with the target receptor upon light irradiation. ANQX thus irreversibly inhibits AMPA receptors and forms an irreversible bond to the active site of the AMPA receptor. This allows fast silencing of cell surface AMPA receptors and real time analysis of AMPA receptor trafficking and lateral diffusion.

Photoactive compounds like ANQX represent a new approach in receptor research, since the compound´s effect can be precisely directed spatio-temporally: low concentration of the compound triggers no effect throughout the sample, while the directed light induces a strong localized effect restricted to the irradiated area. ANQX can be covalently crosslinked to AMPA receptors by directed light, however, precise quantification and localization of the crosslinked complexes cannot be performed. Although it would be extremely important to detect the formation of covalent complexes to identify the specific regions where the AMPA receptor is inhibited, quantify the antagonist effect and follow the stability of the complex.

The recently developed ABQX (Azidobenzoquinoxaline) is similar to ANQX in terms of AMPA receptor inhibition and photoactivity, while it provides a fluorescent signal that allows controlling inhibition efficiency, quantification, localization and stability.

Measurement of ABQX fluorescence allows performing unprecedented experiments in learning and memory research. Using the Molecular Tattoo method, even a single synapse can be selectively modified in the two-photon microscope. The fluorescent signal of ABQX allows the localization of AMPA receptors without any further staining or fluorescent signal, thus these experiments can be performed in genetically unmodified biological samples. The patent application filed regarding this invention also involves the chemical synthesis process of ABQX.

Above all, those institutions might be interested in different possible type of cooperation which perform research of learning and memory functioning.

The Hungarian university possesses the technology and they consider many models of co-operation: licensing patent, patent application, technical cooperation, joint venture, or further R-Y-D. If it suits best to the needs, the university also considers creating joint venture for commercialization.
Advantages and Innovations:
ABQX molecule was developed from the non-fluorescent 6-azido-7-nitro-1,4-dihydroquinoxaline-2,3-dione (ANQX) molecule, which was detected in 2004 and have been used ever since in a number of researches in order to selectively modulate the operation of AMPA (alfa-amino-3-hidroxi-5-metil-4-izoxazolpropion-acid) receptors by directed light. These receptors play a key role in the development of synaptic plasticity, therefore in the basic processes of memory and learning.

Previously described ANQX is used in synaptic plasticity research and can be covalently crosslinked to AMPA receptors by directed light. However, precise quantification and localization of the crosslinked complexes cannot be performed, which would be extremely important especially in subcellular region treatments. ABQX answers this need by providing a fluorescent signal that allows controlling inhibition efficiency, quantification, localization and stability, performing unprecedented experiments in learning and memory research.
Stage of Development:
Available for demonstration
IPs:
Patent(s) applied for but not yet granted

Partner sought

Type and Role of Partner Sought:
The university owning the technology considers many models of co-operation in the following order of importance: licensing patent or patent application, technical cooperation, joint venture, or further R-Y-D with partners - preferably which are specialized in synaptic plasticity, learning and memory research and /or R-Y-D houses with the right technical background.

Client

Type and Size of Client:
University
Already Engaged in Trans-National Cooperation:
Si
Languages Spoken:
English
Hungarian

Keywords

Technology Keywords:
06002002 Biología celular y molecular
06002005 Ingeniería genética