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Método universal de inmovilización de enzimas

Resumen

Tipo:
Oferta Tecnológica
Referencia:
TOES20151008006
Publicado:
02/11/2015
Caducidad:
01/11/2016
Resumen:
Un centro de investigación español ha diseñado un nuevo método universal de inmovilización de enzimas que puede aplicarse independientemente de su tamaño o naturaleza. El soporte utilizado es un marco metalorgánico que atrapa la proteína en su estructura sólida. El método es sencillo y económico y da lugar a biocatalizadores sólidos con bajas pérdidas de actividad. El método permite una alta carga de enzima en el soporte (hasta un 15%) reteniendo su actividad catalítica casi en su totalidad. Se buscan fabricantes de enzimas y empresas químicas interesadas en la utilización de este método de inmovilización para sus enzimas bajo licencia de patente.

Details

Tittle:
Universal method for enzyme immobilization
Summary:
A Spanish public research organisation has designed a novel universal method for the immobilisation of enzymes which can be applied with no dependence of its size or its nature. The supports used are metal organic framework (MOFs) that trap the protein into their solid structure. The method is simple and cheap and leads to solid biocatalyst with slight losses of their activity. Manufacturers of enzymes and chemical companies interested in the use of this method, under patent license, are sought.
Description:
Enzyme immobilisation allows its transformation to a heterogeneous catalyst, facilitating therefore its removal from the reaction medium and its reuse in subsequent reaction cycles. However this is usually a complex process that varies extraordinary depending on the solid support and from one enzyme to other.
This invention consists on a novel method for enzyme immobilization that can be used with any enzyme in a simple and cheap way. The enzymes are immobilized "in-situ" at ambient temperature by adding them into the reaction mixture used for the synthesis of the metal-organic framework (MOF) structure. These materials are formed by a crystalline network of metal ions coordinated to organic molecules. The inter-crystalline mesoporous of the MOF structure, formed by the aggregation or the agglomeration of its nano-crystalline particles, leads to void volumes, stable and highly ordered, where the enzymes are entrapped "in-situ". These mesoporous can be tailored depending on the experimental conditions of the synthesis or even depending on the enzyme that can act as a template, making the MOF structure grown around it.
Some immobilization test where carried out with the enzyme beta-glucosidase from Aspergillus niger into a two MOF composed of aluminum and 2-aminotereftalic acid and other composed of magnesium and N,N-dimetilformammide. They lead to heterogeneous biocatalysts with catalytic activities up to 94 U (transformed micro mol/ min) per gram of solid material.
The Spanish research center is looking for chemical companies or enzyme producers that can be interested in the use of this procedure for the immobilization of their biocatalysts, under a patent license. The method has only been tested at a laboratory scale, but it can be easily adapted to an industrial scale to produce enough amounts of solid biocatalyst.
The Spanish public entity has broad experience in the development of novel catalyst for innovative industrial processes and in the development and optimization of chemical reactions activated by biocatalyst.
Advantages and Innovations:
· The material used as solid support shows a vast diversity of structures, which allows the entrapment of a wide range of enzyme sizes.
· MOFs materials are considerably cheaper than the supports commonly used for enzyme immobilization.
· The method allows a high load of the enzyme into the solid (up to 15% w/w) retaining most of its catalytic activity (up to three times more activity compared with other in-situ methods).
· The entrapped enzyme shows higher stability than the free one.
· The method can be applicable to other biomolecules.
· It can be carried in aqueous media or with organic solvents.
Stage of Development:
Under development/lab tested
IPs:
Patent(s) applied for but not yet granted

Partner sought

Type and Role of Partner Sought:
The Spanish research center is looking for chemical companies or enzyme producers that can be interested in the use of this procedure for the immobilization of their biocatalysts, under a patent license.

Client

Type and Size of Client:
R&D Institution
Already Engaged in Trans-National Cooperation:
No
Languages Spoken:
English
Spanish

Keywords

Technology Keywords:
06006012 Bioprocesos
06004 Micro y nanotecnología relacionada con las ciencias biológicas
02007024 Nanomaterials
06002003 Tecnología de enzimas