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Obtención de extractos de liquen y su uso para mejorar la recuperación de microorganismos asociados a líquenes


Oferta Tecnológica
Un grupo de investigación español ha obtenido un nuevo tipo de extracto de liquen y un método para mejorar el aislamiento cualitativo y cuantitativo de nuevos microorganismos asociados a líquenes. La tecnología, pendiente de patente, es especialmente útil para obtener microorganismos asociados a líquenes sin cultivar que no crecen en cultivos estándar. Se buscan empresas de biotecnología, agricultura o microbiología con el fin de establecer acuerdos de licencia o cooperación técnica.


Obtaining lichen extracts and its use to improve the recovery of lichen-associated microorganisms
Spanish researchers have obtained a new type of lichen extract and a method to improve the qualitative and quantitative isolation of novel microorganisms associated with lichens. The technology, which is patent pending, is especially useful to obtain uncultured lichen-associated microorganisms that do not grow on standard culture media. The researchers are looking for bio-technology, agriculture and/or microbiology companies for license or technical collaboration agreements.
The isolation and identification of novel microorganisms is of great interest to the industry in general for their multiple applications. Therefore, there is a constant need to explore new sources of microorganisms. Recently, different microbial communities associated with lichens have been identified, which makes lichens an important source of new microorganisms. However, to date, this potential source of novel microorganisms has not been appropriately exploited yet because of the difficulties in isolating and obtaining microorganisms associated with lichens. This is mainly due to the use of standard synthetic culture media which do not reproduce the complex nutritive conditions of lichens, conditions which also depend on the species, population and habitat of the lichen used. The present technology overcomes these limitations.

Spanish researchers have developed a novel lichen extract and a method to improve the qualitative and quantitative isolation of microorganisms associated with lichens. The lichenic extract obtained from samples of a particular lichen species is added to the culture medium used. The use of the novel lichenic extract is combined with the new method, which comprises extensive washing of the lichen samples, thereby eliminating the disinfection step of such samples.

The main applications of the technology can be in various sectors of biotechnology, agriculture, agrofood, aquaculture, environment and health science, wherein the discovery of novel microorganisms are always of great interest, for example:

Agriculture, Aquaculture and Agrofood (e.g. identification of plant growth promoting microorganisms by providing nutrients, plant hormones, etc.);
- Environment and Bioremediation (identification of microorganisms able to degrade agricultural wastes by producing various hydrolytic enzymes);
- Biomedicine and Pharmacology (e.g. identification of microorganisms able to produce new antimicrobial and anticancer compounds, etc.);
- Food Industry (e.g. identification of new biopolymers);
- Cosmetic Industry (e.g. identification of novel biomolecules);
- Textile Industry (e.g. identification of new natural pigments).

The Figure shows growth comparison of bacterial strains of Pseudevernia furfuracea (L.) Zopf in a solid culture medium with or without lichen extract, and with or without carbon source (M=minimal culture medium; C=carbon source; L=lichen extract). The addition of the novel lichenic extract to culture media together with the use of the new bacterial isolation method allow at least a ten-fold increase in the number of recovered bacteria from lichen thalli,

The Spanish researchers are looking for specific partners of agri-business committed to sustainable agriculture through innovative research and technology for licensing the invention and for technical cooperation agreements (i.e. testing of applications, adaptation to specific needs, production and marketing).
Advantages and Innovations:
The most innovative aspect of this technology is that the novel lichen extracts mimic the complex nutritive conditions of lichens used as a source of novel microorganisms.

The main advantage provided by the technology is to significantly facilitate the isolation and increase the number (at least 10 times) and diversity of microorganisms associated with lichens, with the identification of novel microbial strains with varied technological applications to discover.
Stage of Development:
Under development/lab tested
Patent(s) applied for but not yet granted
CommeR Statunts Regarding IPR Status:
Spanish patent

Partner sought

Type and Role of Partner Sought:
- Type of partner sought: Enterprise
- Specific area of activity of the partner: agri-business committed to sustainable agriculture through innovative research and technology
- Task to be performed: licensing the invention, testing of applications, adaptation to specific needs, production and marketing.


Type and Size of Client:
Already Engaged in Trans-National Cooperation:
Languages Spoken:


Technology Keywords:
06002008 Microbiología